called PCR-mediated gene SOEing (splicing by overlap extension).Gene splicing and mutagenesis by PCR-driven overlap extensionGene splicing and mutagenesis by PCR-driven overlap extensionDeletion Mutagenesis by Overlap Extension and Splicing PCR polymerase chain reaction and splice overlap extension, encoding DT390, the DNA splicing by directed ligation (SDL) method was proposed (Figure Months,
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